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Lk pd 2562 bam header #1518

Merged
merged 6 commits into from
Feb 26, 2025
Merged

Lk pd 2562 bam header #1518

merged 6 commits into from
Feb 26, 2025

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ekiernan
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This PR adds the cloud path to the reference files used for alignment to the BAM headers for Multiome, Pairedtag, Optimus and ATAC. The resulting header looks like the following:

@CO	user command line: STAR --soloType Droplet --soloStrand Forward --runThreadN 8 --genomeDir genome_reference --readFilesIn /cromwell_root/fc-cddd72b5-323c-495c-9557-5057fff0275a/submissions/intermediates/759925b5-00de-45bd-96d9-8384740bd7e9/TestOptimus/743ea945-b6f4-4ca5-9094-186a4a7c8feb/call-Optimus/Optimus/76860243-c175-4024-95bf-9153f8845e50/call-SplitFastq/cacheCopy/glob-ff9a5e75bc9701a883ca32428a133843/fastq_R2_0.fastq.gz /cromwell_root/fc-cddd72b5-323c-495c-9557-5057fff0275a/submissions/intermediates/759925b5-00de-45bd-96d9-8384740bd7e9/TestOptimus/743ea945-b6f4-4ca5-9094-186a4a7c8feb/call-Optimus/Optimus/76860243-c175-4024-95bf-9153f8845e50/call-SplitFastq/cacheCopy/glob-5b3386a102763e9e23988d2e220cbc9d/fastq_R1_0.fastq.gz --readFilesCommand "gunzip -c" --soloCBwhitelist /cromwell_root/gcp-public-data--broad-references/RNA/resources/3M-febrary-2018.txt --soloUMIlen 12 --soloCBlen 16 --soloFeatures Gene --clipAdapterType CellRanger4 --outFilterScoreMin 30 --soloCBmatchWLtype 1MM_multi --soloUMIdedup 1MM_CR --outSAMtype BAM SortedByCoordinate --outSAMattributes UB UR UY CR CB CY NH GX GN sF cN --soloBarcodeReadLength 0 --soloCellReadStats Standard --soloMultiMappers Uniform --soloUMIfiltering MultiGeneUMI_CR --soloCellFilter EmptyDrops_CR
@CO	Reference genome used: gs://gcp-public-data--broad-references/hg38/v0/star/v2_7_10a/modified_star2.7.10a-Human-GENCODE-build-GRCh38-43.tar

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Remember to squash merge!

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🔍Version Validation Results:

Comparing versions and changelogs for pipelines that differ from the versions on 'origin/staging':
All WDLs and changelog files appear to be valid for this release.

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🔍Changelog Validation Results:

Comparing changelogs for pipelines that differ from the versions on 'origin/develop':
All changelog files are valid for this release.

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@nikellepetrillo nikellepetrillo left a comment

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looks good!

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Remember to squash merge!

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🔍Version Validation Results:

Comparing versions and changelogs for pipelines that differ from the versions on 'origin/staging':
All WDLs and changelog files appear to be valid for this release.

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🔍Changelog Validation Results:

Comparing changelogs for pipelines that differ from the versions on 'origin/develop':
All changelog files are valid for this release.

@ekiernan
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TestPairedTag, TestOptimus, and TestMultiome all passed in Terra workspace

@ekiernan ekiernan merged commit 905d03e into develop Feb 26, 2025
13 of 17 checks passed
@ekiernan ekiernan deleted the lk-PD-2562-bam-header branch February 26, 2025 13:42
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4 participants