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PD-2483 #1182

Merged
merged 4 commits into from
Jan 30, 2024
Merged

PD-2483 #1182

merged 4 commits into from
Jan 30, 2024

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rsc3
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@rsc3 rsc3 commented Jan 24, 2024

Description

Update Multiome metrics with aligner_metrics from Optimus

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Checklist

If you can answer "yes" to the following items, please add a checkmark next to the appropriate checklist item(s) and notify our WARP documentation team by tagging either @ekiernan or @kayleemathews in a comment on this PR.

  • Did you add inputs, outputs, or tasks to a workflow?
  • Did you modify, delete or move: file paths, file names, input names, output names, or task names?
  • If you made a changelog update, did you update the pipeline version number?

@dsde-jenkins
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Can one of the admins verify this patch?

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@dsde-jenkins
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Can one of the admins verify this patch?

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Remember to squash merge!

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Remember to squash merge!

@kayleemathews
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@rsc3 This will require some minor documentation updates. I can take a look and make those updates today!

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Remember to squash merge!

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@kayleemathews kayleemathews left a comment

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Docs are updated accordingly - LGTM!

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@khajoue2 khajoue2 left a comment

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looks good

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@ekiernan ekiernan left a comment

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Looks good! Thank you!

@nikellepetrillo
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add to whitelist

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Remember to squash merge!

@rsc3 rsc3 merged commit 38200f5 into develop Jan 30, 2024
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@rsc3 rsc3 deleted the rc-2483-promotemultiome branch January 30, 2024 15:18
nikellepetrillo added a commit that referenced this pull request Feb 5, 2024
* update paths to input files

* update jg sample map

* Km buildindices docs (#1158)

* add buildindices overview doc and diagram

* Km rnawithumis and ss2 doc updates (#1157)

* update rnawithumis overview

* Update rna-with-umis.methods.md

* Update rna-with-umis.methods.md

* update multi-snSS2 readme

* Update multi_snss2.methods.md

* Update multi_snss2.methods.md

* update multi-snSS2 docs

* update SS2 overview doc

* fix python script link

* Lk pd2448 upstools (#1150)

Added paired-tag wrapper and demultiplexing task

* PD-2435 Test bwa-mem2 step and run Intel distributed BWA-MEM2  (#1147)

* Lk pd2453 add bb tag (#1161)

Added option to incorporate BB tag in BAM and use it in SnapATAC2 software.

* km paired-tag docs (#1165)

* update overview docs

- update pipeline version numbers in Multiome and Optimus Overview docs

* update multi-SS2 overview doc

* Update smart-seq2.methods.md

* update multi-SS2 methods doc

* Update doc_style.md

* add paired-tag overview doc

* Update website/docs/Pipelines/PairedTag_Pipeline/README.md

Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

* Apply suggestions from LK doc review

Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

---------

Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

* Np jprb pd 2353 multiple star rsolo align (#1164)

* integrate multiple soloFeatures

* updating counting_mode definition

* logic

* count exons is true

* count exons is true

* fix the logic

* count exons false

* count exons false

* echos

* echos

* echos

* rearrange logic

* rearrange

* testing

* testing

* testing

* count exons true

* switch counting mode order

* try running in scrna

* clean up

* snrna countexons is true

* snrna countexons is true

* snrna countexons is false

* snrna countexons is true

* snrna countexons is false

* cleaning up

* changelogs

* changelogs

* change cpu_platform to Intel Cascade Lake for sci test input

* change cpu_platform to Intel Cascade Lake for sci test input

* Update pipelines/skylab/smartseq2_single_nucleus_multisample/MultiSampleSmartSeq2SingleNucleus.changelog.md

Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

* Update pipelines/skylab/multiome/Multiome.changelog.md

Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

* Update pipelines/skylab/optimus/Optimus.changelog.md

Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

* Update pipelines/skylab/paired_tag/PairedTag.changelog.md

Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

* Update pipelines/skylab/slideseq/SlideSeq.changelog.md

Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

---------

Co-authored-by: Juan Pablo Ramos Barroso <jpramosbarroso@gmail.com>
Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

* Np update multiome sci test (#1167)

* add summary task

* change cpu_platform to Intel Cascade Lake for sci test input

* change cpu_platform to Intel Cascade Lake for sci test input

* change cpu_platform to Intel Cascade Lake for sci test input

* Update VerifyTasks.wdl

* Update VerifyTasks.wdl

* PD-2422 BICAN_Optimus_2nymxis_Oct_2023 (#1152)

* Np multimapper param starsolo (#1172)

* add summary task

* add multimapper option

* update optimus plumbing for ease of testing

* add echos

* add to test

* remove some echoes

* make mouse snrna json go back to what is in dev

* make mouse snrna json go back to what is in dev

* add as outputs

* typo

* changelogs

* changelogs

* changelogs

* update pipeline docs

* optional output

* optional output

* optional output

* optional output

* docs

* docs

* docs

* Update website/docs/Pipelines/Optimus_Pipeline/README.md

Co-authored-by: Kaylee Mathews <95316074+kayleemathews@users.noreply.github.com>

* remove optional input to tests

---------

Co-authored-by: kayleemathews <kmathews@broadinstitute.org>
Co-authored-by: Kaylee Mathews <95316074+kayleemathews@users.noreply.github.com>

* added exit code to CompareTabix (#1174)

Updated the CompareTabix task in the Verify tasks

* Lk pd 2452 add read length check (#1171)

* adding read2 length and barcode orientation check task

* Lk pd 2464 batch methylome (#1181)

Added scatter and preemptibles to snM3C

* Np edit resources needed for bwa task / add logic to compareBams (#1183)

* add summary task

* get a bigger machine

* more memory

* trying out east1

* go back to central and decrease mem and threads

* try different zones

* make machine smaller

* smaller cpu

* smaller cpu

* more mem

* more mem

* 2000 disk

* more mem compare bams

* more mem compare bams

* more mem compare bams

* no zones

* more mem in comparebams

* more mem in comparebams

* 725000

* 825000

* 725

* max records 3000000

* max records 3000000

* add logic to fail fast if bams differ in size by 200 mb

* Update VerifyTasks.wdl

* PD-2483 (#1182)

* rc-2483

* update changelog

* Update README.md

---------

Co-authored-by: kayleemathews <kmathews@broadinstitute.org>
Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>

* PD-2476: Add task before fastqprocess to find number of splits (#1178)

* removed space (#1184)

Removed space from Verify Tabix task

* Lk pd 2455 pairedtag parsebarcodes (#1186)

Added a task to parse cell barcodes from sample barcodes

* Np move snm3c from beta pipelines  (#1185)

* add summary task

* move CondensedSnm3C.wdl out of beta

* update batch numbers

* add sorting to the compare compressed text files

* changelogs and versions

* revert batch change

* batch change

* batch change

* Km snm3c overview doc (#1179)

* Update README.md

* Update README.md

* Update README.md

* Update README.md

* Update README.md

* Update website/docs/Pipelines/snM3C/README.md

Co-authored-by: Nikelle Petrillo <38223776+nikellepetrillo@users.noreply.github.com>

* Update README.md

* fix num_downstr_bases description

* Update README.md

---------

Co-authored-by: Nikelle Petrillo <38223776+nikellepetrillo@users.noreply.github.com>

* Np fix snm3c test (#1190)

* add summary task

* batch change in test wdl

---------

Co-authored-by: Kaylee Mathews <95316074+kayleemathews@users.noreply.github.com>
Co-authored-by: ekiernan <55763654+ekiernan@users.noreply.github.com>
Co-authored-by: aawdeh <awdeh@broadinstitute.org>
Co-authored-by: Nikelle Petrillo <38223776+nikellepetrillo@users.noreply.github.com>
Co-authored-by: Juan Pablo Ramos Barroso <jpramosbarroso@gmail.com>
Co-authored-by: kayleemathews <kmathews@broadinstitute.org>
Co-authored-by: Robert Sidney Cox III <sidcox@broadinstitute.org>
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6 participants