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Merge pull request #11 from EMBL-EBI-GCA/jan_2015_release
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Jan 2015 release
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davidrichardson committed Jan 28, 2015
2 parents eea6a17 + 910cb10 commit 8d940b2
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2 changes: 1 addition & 1 deletion app/data/ERX142089.json
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1 change: 1 addition & 0 deletions app/data/ERX142090.json
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{"archive_url":"https://www.ebi.ac.uk/ega/datasets/EGAD00001001184","experiment":{"EXPERIMENT_TYPE":"flRNA-seq","EXPERIMENT_ID":"EGAX00001065546","attributes":{"CDNA_PREPARATION_PCR_NUMBER_CYCLES":["20"],"EXPERIMENT_TYPE":["flRNA-seq"],"LIBRARY_GENERATION_PCR_POLYMERASE_TYPE":["Phusion High-Fidelity DNA Polymerase (Finnzymes)"],"LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE":["AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT"],"LIBRARY_GENERATION_CDNA_FRAGMENTATION":["Full-length cDNA was sheared using Bioruptor sonicator, conditions: sample volume 75 ul, 30 high power cycles of 30”"],"LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM":["30\" 98ºC, [40\" 98ºC, 30\" 65ºC, 30\" 72ºC]14 cycles, 5' 72ºC, hold 4ºC"],"LIBRARY_GENERATION_PCR_TEMPLATE_CONC":["NA"],"CDNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL":["SMARTer Ultra Low RNA Kit for Illumina Sequencing, Clontech Laboratories version:PR0X3693"],"LIBRARY_GENERATION_PCR_PRIMER_CONC":["25µM"],"LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE":["CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT"],"LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL":["PCR Purification Kit using MinElute column (Qiagen)"],"LIBRARY_GENERATION_PROTOCOL":["30ul Sheared cDNA was end repaired and a 3' A overhangh was created using Klenow exo (3’ to 5’ exo minus) ( (New England Biolabs)) for 30 minutes at 37ºC. 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