You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hello Lex,
I'm adjusting your Gene_mining.slurm script from S19_genome_mining_and_gene_identification/scripts
to mine mammal immune genes but I got stuck at the extract_fasta step.
I assume this command merges all the unitig reads(containing predicted ORFs) with the same prefix in the file name, but I was wondering how it merges unitig IDs.
Is extract_fasta a part of some particular package or you maybe have it stuck somewhere?
I would appreciate a lot if you could share that piece of code.
Thank you!
Lorena
The text was updated successfully, but these errors were encountered:
it seems it's been awhile since you've been working on this script :D
so I wanted to suggest a somewhat easier solution if you don't mind,
and that is to please send me an example of the output file in the following step if possible:
for f in ORFs/*; do \
extract_fasta $f \
>> ${f%_splitted_utg_reads_???}"_merged_ORFs" \
; done
cd ORFs
mkdir ORFs_FASTA
mv *_ORFs ORFs_FASTA
echo "6) Utg-ORFs predicted and parsed" \
>> ~/Progressreports_2015/Progress_$FISH
so I could perhaps reconstruct the extract_fasta command and write it on my own based on one of the output files with the "_merged_ORFs" suffix.
Hello Lex,
I'm adjusting your Gene_mining.slurm script from
S19_genome_mining_and_gene_identification/scripts
to mine mammal immune genes but I got stuck at the
extract_fasta
step.I assume this command merges all the unitig reads(containing predicted ORFs) with the same prefix in the file name, but I was wondering how it merges unitig IDs.
Is
extract_fasta
a part of some particular package or you maybe have it stuck somewhere?I would appreciate a lot if you could share that piece of code.
Thank you!
Lorena
The text was updated successfully, but these errors were encountered: