diff --git a/server/src/main/java/org/opensearch/index/shard/RemoteStoreRefreshListener.java b/server/src/main/java/org/opensearch/index/shard/RemoteStoreRefreshListener.java
index 1a8911c657fac..7010b28090b32 100644
--- a/server/src/main/java/org/opensearch/index/shard/RemoteStoreRefreshListener.java
+++ b/server/src/main/java/org/opensearch/index/shard/RemoteStoreRefreshListener.java
@@ -460,7 +460,7 @@ private void uploadNewSegments(
                 batchUploadListener.onFailure(ex);
             });
             statsListener.beforeUpload(src);
-            remoteDirectory.copyFrom(storeDirectory, src, IOContext.READONCE, aggregatedListener, isLowPriorityUpload());
+            remoteDirectory.copyFrom(storeDirectory, src, IOContext.DEFAULT, aggregatedListener, isLowPriorityUpload());
         }
     }
 
diff --git a/server/src/main/java/org/opensearch/index/store/RemoteDirectory.java b/server/src/main/java/org/opensearch/index/store/RemoteDirectory.java
index aa856aa22313d..726e94e6d9448 100644
--- a/server/src/main/java/org/opensearch/index/store/RemoteDirectory.java
+++ b/server/src/main/java/org/opensearch/index/store/RemoteDirectory.java
@@ -357,6 +357,7 @@ private void uploadBlob(
         ActionListener<Void> listener,
         boolean lowPriorityUpload
     ) throws Exception {
+        assert ioContext != IOContext.READONCE : "Remote upload will fail with IoContext.READONCE";
         long expectedChecksum = calculateChecksumOfChecksum(from, src);
         long contentLength;
         try (IndexInput indexInput = from.openInput(src, ioContext)) {
diff --git a/server/src/test/java/org/opensearch/index/store/RemoteDirectoryTests.java b/server/src/test/java/org/opensearch/index/store/RemoteDirectoryTests.java
index ed79a2b0bd8e4..b31acdffbded9 100644
--- a/server/src/test/java/org/opensearch/index/store/RemoteDirectoryTests.java
+++ b/server/src/test/java/org/opensearch/index/store/RemoteDirectoryTests.java
@@ -94,7 +94,7 @@ public void testCopyFrom() throws IOException, InterruptedException {
             storeDirectory,
             filename,
             filename,
-            IOContext.READ,
+            IOContext.DEFAULT,
             () -> postUploadInvoked.set(true),
             new ActionListener<>() {
                 @Override
@@ -132,7 +132,7 @@ public void testCopyFromWithException() throws IOException, InterruptedException
             storeDirectory,
             filename,
             filename,
-            IOContext.READ,
+            IOContext.DEFAULT,
             () -> postUploadInvoked.set(true),
             new ActionListener<>() {
                 @Override
diff --git a/test/framework/src/main/java/org/opensearch/test/store/MockFSDirectoryFactory.java b/test/framework/src/main/java/org/opensearch/test/store/MockFSDirectoryFactory.java
index 9c42ea2672601..d8279170ddd92 100644
--- a/test/framework/src/main/java/org/opensearch/test/store/MockFSDirectoryFactory.java
+++ b/test/framework/src/main/java/org/opensearch/test/store/MockFSDirectoryFactory.java
@@ -36,7 +36,10 @@
 
 import org.apache.logging.log4j.Logger;
 import org.apache.lucene.index.CheckIndex;
+import org.apache.lucene.index.IndexFileNames;
 import org.apache.lucene.store.Directory;
+import org.apache.lucene.store.IOContext;
+import org.apache.lucene.store.IndexInput;
 import org.apache.lucene.store.LockObtainFailedException;
 import org.apache.lucene.tests.store.BaseDirectoryWrapper;
 import org.apache.lucene.tests.store.MockDirectoryWrapper;
@@ -203,6 +206,19 @@ public synchronized void crash() throws IOException {
         public Set<String> getPendingDeletions() throws IOException {
             return in.getPendingDeletions();
         }
+
+        // In remote store feature, the upload flow is async and IndexInput can be opened and closed
+        // by different threads, so we always use IOContext.DEFAULT.
+        // But MockDirectoryWrapper throws an exception if segments_N fil is opened with any IOContext other than READONCE.
+        // Following change is temporary override to avoid the test failures. We should fix the multiple thread access
+        // in remote store upload flow.
+        @Override
+        public synchronized IndexInput openInput(String name, IOContext context) throws IOException {
+            if (name.startsWith(IndexFileNames.SEGMENTS)) {
+                context = IOContext.READONCE;
+            }
+            return super.openInput(name, context);
+        }
     }
 
     static final class CloseableDirectory implements Closeable {