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Hello,
I am aware that there is an open issue (#336) regarding this matter, but I still wanted to update the problem. Despite trying the codes below, I am unable to obtain the outputs from MACS2. Additionally, using the -genome parameter results in the following error: 'taging foreign file: s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index ERROR ~ Error executing process > 'NFCORE_CHIPSEQ:CHIPSEQ:ALIGN_BOWTIE2:BOWTIE2_ALIGN (1)' Caused by: Not a valid S3 file system provider file attribute view: java.nio.file.attribute.BasicWithKeyFileAttributeView -- Check '.nextflow.log' file for details -[nf-core/chipseq] Pipeline completed with errors- WARN: Killing running tasks (1)'. Can you help me with this?
Best regards
See the answer here regarding the macs issue.
Concerning the aws download problem, you can bypass the issue by manually downloading the iGenomes reference file and providing it to the pipeline using the --bowtie2_index parameter.
If you don't want to pre-download the file, from slacks exchanges this error is many times caused due to not be using a suitable java version, so you can also try to update java (Nextflow needs java 11 or later)
Description of the bug
Hello,
I am aware that there is an open issue (#336) regarding this matter, but I still wanted to update the problem. Despite trying the codes below, I am unable to obtain the outputs from MACS2. Additionally, using the -genome parameter results in the following error: 'taging foreign file: s3://ngi-igenomes/igenomes/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index ERROR ~ Error executing process > 'NFCORE_CHIPSEQ:CHIPSEQ:ALIGN_BOWTIE2:BOWTIE2_ALIGN (1)' Caused by: Not a valid S3 file system provider file attribute view: java.nio.file.attribute.BasicWithKeyFileAttributeView -- Check '.nextflow.log' file for details -[nf-core/chipseq] Pipeline completed with errors- WARN: Killing running tasks (1)'. Can you help me with this?
Best regards
Command used and terminal output
Relevant files
No response
System information
Version of nf-core/chipseq 2.0.0
Hardware HPC
Executor slurm
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