example.p3

SEQUENCE_ID=example
SEQUENCE_TEMPLATE=ACATGCACACATGANAGTCTACCCCAGCATCTCTGAAATACTCACTGTTATCTCACATGGCACAGAAGCCAGAGGGTTTATATCTGATCATAAAAAGATGCAATTATGAAGCAGCACAGAAGCTACATCCNTCACAGTTGCTGTTTTTCTTGGTGATTCTTTTGGTTTGAGTGATTAAAAAAAACAAAAACACAACACTAGGAAATTGCAATCAGCTTGCATTAGGAAAACTAAACAATTGTTCTACTTTTCTAATTATCCGGAGATCTGTTCCTTTTTTTTTTTGCTGTTTTTGTTTGTTTGTTTGTTTGTTTGTTCCTATGTGCATATTTTAGAAAAAATAAGGTATACAACCAGCACTGTGTAAGGAAATTGACAAAACGGGGAGATGTCAGTTTTGTTTTGTTTTAATAATGTAAAAAAATTAGAGAAGTTTAGGGAAGGAATGAAGTTTGCTTTGAGATACCACACACTTCCTAAAGGAACAGAATCAAAGTTATTTAGAAAAAAGCAGCTATCCATGCTTACAGAAAGGACATGTGAGAAATTTATACTGCTCATTACATACACATGATTTGTTTTAAATCTCTTCCTCCAATCTTCAGCAAGTTAGAAGC
SEQUENCE_TARGET=37,21
PRIMER_TASK=pick_pcr_primers
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_DIVALENT=1.5
PRIMER_DNTP_CONC=0.125
PRIMER_THERMODYNAMIC_ALIGNMENT=1
PRIMER_MAX_POLY_X=4
PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=50-200
PRIMER_MIN_TM=55.
PRIMER_MAX_TM=65.
PRIMER_OPT_TM=62.
PRIMER_PAIR_MAX_DIFF_TM=5.
PRIMER_OPT_SIZE=19
PRIMER_MIN_SIZE=16
PRIMER_MIN_GC=30.0
PRIMER_OPT_GC_PERCENT=50.0
PRIMER_MAX_GC=70.0
PRIMER_GC_CLAMP=1
PRIMER_MAX_SELF_ANY_TH=45.
PRIMER_PAIR_MAX_COMPL_ANY_TH=45.
PRIMER_MAX_SELF_END_TH=40.
PRIMER_PAIR_MAX_COMPL_END_TH=40.
PRIMER_MAX_HAIRPIN_TH=24.
PRIMER_PAIR_MAX_HAIRPIN_TH=24.
PRIMER_MAX_END_STABILITY=8.0
PRIMER_LOWERCASE_MASKING=1
PRIMER_NUM_RETURN=4
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/Users/bcf/Bin/primer3_config/
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