diff --git a/anta/tests/stp.py b/anta/tests/stp.py
index 93a0d2e39..7a625ff8e 100644
--- a/anta/tests/stp.py
+++ b/anta/tests/stp.py
@@ -309,3 +309,67 @@ def test(self) -> None:
             self.result.is_failure(f"The following STP topologies are not configured or number of changes not within the threshold:\n{failures}")
         else:
             self.result.is_success()
+
+
+class VerifySTPDisabledVlans(AntaTest):
+    """Verifies the STP disabled VLAN(s).
+
+    This test performs the following checks:
+
+        1. Verifies that the STP is configured.
+        2. Verifies that the specified VLAN(s) exist on the device.
+        3. Verifies that the STP is disabled for the specified VLAN(s).
+
+    Expected Results
+    ----------------
+    * Success: The test will pass if all of the following conditions are met:
+        - STP is properly configured on the device.
+        - The specified VLAN(s) exist on the device.
+        - STP is confirmed to be disabled for all the specified VLAN(s).
+    * Failure: The test will fail if any of the following condition is met:
+        - STP is not configured on the device.
+        - The specified VLAN(s) do not exist on the device.
+        - STP is enabled for any of the specified VLAN(s).
+
+    Examples
+    --------
+    ```yaml
+    anta.tests.stp:
+      - VerifySTPDisabledVlans:
+            vlans:
+              - 6
+              - 4094
+    ```
+    """
+
+    categories: ClassVar[list[str]] = ["stp"]
+    commands: ClassVar[list[AntaCommand | AntaTemplate]] = [AntaCommand(command="show spanning-tree vlan detail", revision=1)]
+
+    class Input(AntaTest.Input):
+        """Input model for the VerifySTPDisabledVlans test."""
+
+        vlans: list[Vlan]
+        """List of STP disabled VLAN(s)."""
+
+    @AntaTest.anta_test
+    def test(self) -> None:
+        """Main test function for VerifySTPDisabledVlans."""
+        self.result.is_success()
+
+        command_output = self.instance_commands[0].json_output
+        stp_vlan_instances = command_output.get("spanningTreeVlanInstances", {})
+
+        # If the spanningTreeVlanInstances detail are not found in the command output, the test fails.
+        if not stp_vlan_instances:
+            self.result.is_failure("STP is not configured")
+            return
+
+        actual_vlans = list(stp_vlan_instances)
+        # If the specified VLAN is not present on the device, STP is enabled for the VLAN(s), test fails.
+        for vlan in self.inputs.vlans:
+            if str(vlan) not in actual_vlans:
+                self.result.is_failure(f"VLAN: {vlan} - Not configured")
+                continue
+
+            if stp_vlan_instances.get(str(vlan)):
+                self.result.is_failure(f"VLAN: {vlan} - STP is enabled")
diff --git a/examples/tests.yaml b/examples/tests.yaml
index e22acf49a..6e1fc5b85 100644
--- a/examples/tests.yaml
+++ b/examples/tests.yaml
@@ -749,6 +749,11 @@ anta.tests.stp:
       # Verifies there is no STP blocked ports.
   - VerifySTPCounters:
       # Verifies there is no errors in STP BPDU packets.
+  - VerifySTPDisabledVlans:
+      # Verifies the STP disabled VLAN(s).
+      vlans:
+        - 6
+        - 4094
   - VerifySTPForwardingPorts:
       # Verifies that all interfaces are forwarding for a provided list of VLAN(s).
       vlans:
diff --git a/tests/units/anta_tests/test_stp.py b/tests/units/anta_tests/test_stp.py
index 37422108b..bcd83f11d 100644
--- a/tests/units/anta_tests/test_stp.py
+++ b/tests/units/anta_tests/test_stp.py
@@ -7,7 +7,15 @@
 
 from typing import Any
 
-from anta.tests.stp import VerifySTPBlockedPorts, VerifySTPCounters, VerifySTPForwardingPorts, VerifySTPMode, VerifySTPRootPriority, VerifyStpTopologyChanges
+from anta.tests.stp import (
+    VerifySTPBlockedPorts,
+    VerifySTPCounters,
+    VerifySTPDisabledVlans,
+    VerifySTPForwardingPorts,
+    VerifySTPMode,
+    VerifySTPRootPriority,
+    VerifyStpTopologyChanges,
+)
 from tests.units.anta_tests import test
 
 DATA: list[dict[str, Any]] = [
@@ -486,4 +494,48 @@
         "inputs": {"threshold": 10},
         "expected": {"result": "failure", "messages": ["STP is not configured."]},
     },
+    {
+        "name": "success",
+        "test": VerifySTPDisabledVlans,
+        "eos_data": [{"spanningTreeVlanInstances": {"1": {"spanningTreeVlanInstance": {"protocol": "mstp", "bridge": {"priority": 32768}}}, "6": {}, "4094": {}}}],
+        "inputs": {"vlans": ["6", "4094"]},
+        "expected": {"result": "success"},
+    },
+    {
+        "name": "failure-stp-not-configured",
+        "test": VerifySTPDisabledVlans,
+        "eos_data": [{"spanningTreeVlanInstances": {}}],
+        "inputs": {"vlans": ["6", "4094"]},
+        "expected": {"result": "failure", "messages": ["STP is not configured"]},
+    },
+    {
+        "name": "failure-vlans-not-found",
+        "test": VerifySTPDisabledVlans,
+        "eos_data": [
+            {
+                "spanningTreeVlanInstances": {
+                    "1": {"spanningTreeVlanInstance": {"protocol": "mstp", "bridge": {}}},
+                    "6": {"spanningTreeVlanInstance": {"protocol": "mstp", "bridge": {}}},
+                    "4094": {"spanningTreeVlanInstance": {"protocol": "mstp", "bridge": {}}},
+                }
+            }
+        ],
+        "inputs": {"vlans": ["16", "4093"]},
+        "expected": {"result": "failure", "messages": ["VLAN: 16 - Not configured", "VLAN: 4093 - Not configured"]},
+    },
+    {
+        "name": "failure-vlans-enabled",
+        "test": VerifySTPDisabledVlans,
+        "eos_data": [
+            {
+                "spanningTreeVlanInstances": {
+                    "1": {"spanningTreeVlanInstance": {"protocol": "mstp", "bridge": {}}},
+                    "6": {"spanningTreeVlanInstance": {"protocol": "mstp", "bridge": {}}},
+                    "4094": {"spanningTreeVlanInstance": {"protocol": "mstp", "bridge": {}}},
+                }
+            }
+        ],
+        "inputs": {"vlans": ["6", "4094"]},
+        "expected": {"result": "failure", "messages": ["VLAN: 6 - STP is enabled", "VLAN: 4094 - STP is enabled"]},
+    },
 ]